Institute of Microstructure Technology (IMT)


Our particle based method allows us to synthesise very high complexity peptide arrays by combinatorial synthesis and for an unrivalled prize. (more) Within the EU FP7 project PEPLASER, we plan to further develop this new technology up to the level of robust prototype machines, and mate it to bioinformatics ( and and readout tools. The development of the 2nd generation peptide laser printer is led by our cooperation partner Fraunhofer IPA, with engineering mostly done at SME Kemmer Automation, and programming done at Microsystems LTD, Bulgaria and at the Technical University of Varna, Bulgaria. Together, our procedure(s) should boost the field of proteomics in a similar way as the lithographic technologies did with the field of genomics. Central to our novel method are the activated chemical building blocks that are “frozen” within solid amino acid particles. Thereby, we can use a modified colour laser printer to send them to defined addresses on a 2D support, where the particles are simply melted to induce a spatially defined coupling reaction of now freed amino acid derivatives. By repeated printing and melting cycles this simple trick yields high complexity peptide arrays. Based on existing pre-prototypes, (more) we will develop a user friendly peptide laser printer that spatially defined addresses our 20 different amino acid toners in high resolution to a support, and a scanner (, that especially fast and sensitive reads out the large formats delivered by the peptide laser printer. The increased production of amino acid toners and array supports are other bottlenecks in the output of peptide arrays that are tackled in the PEPLASER project. This should allow us to increase the output of individual peptide spots from currently 0,5 Million to >10 Million peptides per month. Finally, to foster a market for high complexity peptide arrays, we will work out paradigmatic applications. (more) One of these applications aims for the comprehensive readout of the different antibodies that patrol the serum of patients suffering from Malaria. (more) Based on user friendly prototype machines, on first paradigmatic application examples for high complexity peptide arrays, and shielded by a strong patent, the participating SMEs will commercialise this new technology. AIMS scientific will commercialise novel solid support materials, PEPperPRINT will commercialise our novel particle based peptide arrays, and AKAtech will commercialise our novel scanner.

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Project partners

PD Dr. Frank Breitling
Phone: +49 721 608-23859
frank breitlingTjz0∂kit edu

Karlsruher Institut für Technologie
Institut für Mikrostrukturtechnik
Hermann-von-Helmholtz-Platz 1
76344 Eggenstein-Leopoldshafen

Michael Farthofer

AKAtech Produktions- und Handels GmbH
Untermühlberg 1
Frankenmarkt 4890

Christa Dobberphul and Ronald Frank

AIMS-Scientific Products GmbH
Inhoffenstraße 7
38124 Braunschweig

Dr. Leszek Rychlewski

BioInfoBank Institute
Limanowskiego 24/a 16
Poznan 60744

Dr. Alois Sonnleitner

Centre for Biomedical Nanotechnology
Upper Austrian Research GmbH
Hafenstrasse 47-51
4020 Linz

PD Dr. Bischoff
Dep. Chip based peptide arrays

German Cancer Research Centre
Im Neuenheimer Feld 280
69120 Heidelberg

Dr. Stefan Güttler

Fraunhofer Institute for Manufacturing Engineering and Automation,
Dep. Information Processing
Hansastrasse Number 27C
80686 München

Dr. Gunter Hagen

KMS Kemmer Automation GmbH
Im Webertal 20
78713 Schramberg-Waldmössingen

Nikolay Nikolov

Microsystems LTD
kv. CHAIKA 15-D-21
Varna 9010

Dr. Volker Stadler

Schlossweg 9
69168 Wiesloch

Dr. Peycho Popov

Technical University of Varna, Department of Physics
Studentska 1
VARNA 9010