Cary Eclipse fluorescence spectrometer

Cary Eclipse

Device name: Cary Eclipse fluorescence spectrometer

Device responsible: Nicolò Baroni

Room: R203

Wavelength range Excitation: 190 - 1100 nm

Wavelength range Emission: 190 - 1100 nm

Dimensions (W x D x H): 560 mm x 560 mm x 250 mm

Getting started-Scan

a. Turn on the device and turn on the computer; don’t start the software immediately.

b. Open the folder on desktop "Cary Eclipse" and choose the task "Scan".

c. Go to scan set-up to put the desired setting (see below).

d. press Ok and then press the start button.

Scanning Set-Up

I Three modes of operation are offered: fluorescence, phosphorescence and bio-/chemi-luminescence.

II Select the measurement to perform: excitation, emission and synchronous (scans with a constant wavelength or wavenumber difference between monochromators to resolve multi-component mixtures).

III Select to scan in wavelength, wavenumber, angstroms or electron volts.

IV Set excitation and starting and ending wavelengths.

V Precise control over how much signal averaging is done and how frequently data points are collected.

I Set excitation and emission filter.

II Set the voltage of the PMT detector to have a better resolution of the low intensity signals.