Background

Pharmaceutical research increasingly depends on microfluidics-based
advanced in-vitro models (AIM) based on sophisticated cultures of cells
and cell aggregates for pre-clinical testing. Since they can use human
cells, AIM are potentially more accurate than animal models. Their introduction
is also driven by the recognised need to reduce animal testing.
Gaining useful information from AIM is a challenge, as analytical techniques
must be at the same time highly informative and keep the cells
alive and functional. Nuclear Magnetic Resonance (NMR) spectroscopy
provides detailed, non-invasive, real-time insights into AIM life processes
but is currently limited to single-sample observation. This project aims to
efficiently analyse multiple samples to enable high-throughput operation.

Goals

The objective of this Master project is to develop and implement a multisample
analysis capability for AIM by NMR, allowing for simultaneous and
detailed monitoring of life processes in multiple in vitro models.

Approach

The project will develop in three phases:

1. Develop and produce a microfluidic insert that integrates with commonly used biological culturing platforms.
2. Design and build a prototype of the insert injector system for integration into an NMR spectrometer.
3. Conduct a proof-of-concept study to showcase the high-throughput capabilities of the system within an NMR spectrometer environment.

It is expected that the successful project leads to at least one publication
in an internationally peer-reviewed journal.

Requirements

You are an ambitious mechanical engineer passionate about product design.
You should possess proficiency in 3D modeling and practical expertise
in programming microcontrollers.

Supervision

Prof. Dr. Marcel Utz (marcel.utz∂kit.edu)
Dr. Sylwia Barker (sylwia.barker∂kit.edu)

Available Start date is negotiable from 1.2.2024